Nowadays different in vitro techniques are employed in order to preserve plant species. Among these, cryopreservation (the conservation of plant material at ultralow temperature, usually at -196 ºC, which is the temperature of liquid nitrogen) is widely employed for long-term storage of plant germplasm. These techniques could complement other ex situ strategies like seed banks or field collections, and are the unique reliable way for long-term preservation of species that are vegetatively propagated or have recalcitrant seeds. Recently, cryopreservation protocols in Tetraclinis articulata and Thymus moroderi explants have been developed.
In vitro conservation; Cryopreservation; Thymus moroderi; Tetraclinis articulata; Vitrification